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The procedure of intracytoplasmic sperm injection is performed under laboratory condition to improve the chances of conception during the procedure of In Vitro Fertilization, for the couples who are suffering from male pertinent infertility factor. Normally, in IVF, the egg is fertilized with almost one lac sperms and out of them one sperm enters the egg on its own. During the ICSI procedure, one single but live, healthy and potential sperm is injected into the egg with the help of a special medical equipment known as micromanipulator. And this is all done under the sublime supervision of IVF specialist and doctor.

Indications of ICSI

  • Very low number of sperms (less then 5 million),very poor motility.

  • Severe teratospermia.
  • Problems with sperm binding to and penetrating the egg.

  • Antisperm antibodies thought to be the cause of infertility.

  • Could not get success with previous standard IVF procedures.

  • When there is low amount of frozen sperm and limited in quality as well

  • When there is certain obstruction (tubal blockage) in male reproductive tract which is not subjected to repair. Under the circumstances, the sperm is retrieved from the epididymis through a microsurgical procedure known as microsurgical epididymal sperm aspiration (MESA), alternatively, it is obtain from the testes by procedure called testicular sperm aspiration (TESA).

  • Males with ejaculatory dysfunction due to certain disease, medical problem or injury in spine.

  • Retrograde ejaculation

ICSI Procedure

The investigations listed by an IVF specialist and the hormonal injections availed in the procedure to produce a significant amount of eggs are similar to the conventional In Vitro Fertilization (IVF). After the eggs are efficiently collected and identified by the embryologist, the Cumulus Oophorus, which is the outer layer of cells around each egg, is removed to view the egg without any obstructions. The eggs should be at a mature stage (M2) during the ICSI procedure. The procedure is thus accomplished with the use of a microscope using micromanipulation devices: micromanipulators, microinjectors, micropipettes. The mature oocyte is stabilized by a holding pipette on the left. Then, from the opposite side, a thin, hollow needle is pierced through the oolema and into the inner part of the oocyte. The needle is loaded with a single active sperm which will be released into the oocyte. On following day after the procedure, the oocyte is then checked for signs of fertilization.

ICSI Main Objective

The main objective of IVF specialist in performing ICSI is to minimize the rise of fertilization failure. At our centre, IVF specialized doctor uses ICSI back up to ensure fertilization in cases of unexplained infertility also. When the number of sperm is minute or has abnormal morphology or motility, it will result in the impairment in the function of the sperm. With ICSI, a single sperm is carefully and precisely placed within the egg.

ICSI Facts

Fertilization occurs in 50% to 80% of injected eggs. A total of 30% of all ICSI cycles performed results in live birth. According to an IVF doctor, younger patients are more probable to achieve favorable results from ICSI. Because ICSI enables infertile couples to achieve pregnancy and become parents of their own genetical child, it is a preferred method that is more prominent than Donor Insemination or Adoption.