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Intracytoplasmic sperm injection (ICSI) is a laboratory procedure developed to help infertile couples undergoing In Vitro Fertilization (IVF) because of male infertility. In the IVF process, each egg is normally mixed with about 100,000 sperms to ensure that one of the active sperms generates its way into the egg. But in ICSI, a single live sperm is injected by an IVF doctor in the egg with the assistance of a special machine called the micromanipulator. And this is all done under the sublime supervision of IVF specialist and doctor.

Indications of ICSI

  • Production of minute amount of sperm.
  • Severe teratospermia.
  • Issue with effective penetration of the egg and sperm binding.
  • Infertility caused by antisperm antibodies.
  • Failure with standard IVF methods.
  • Limitations in the number and quality of the frozen sperm.
  • Obstruction of the male reproductive tract not amenable to repair. Sperm may then be obtained by the IVF specialist from the epididymis by a procedure called microsurgical epididymal sperm aspiration (MESA), or from the testes by testicular sperm aspiration (TESA).
  • Ejaculatory dysfunction in male resulting from spinal disease or injury may persuade doctor to go for ICSI.
  • The IVF specialist may also consider ICSI if there is Retrograde Ejaculation.

ICSI Procedure

The investigations listed by an IVF specialist and the hormonal injections availed in the procedure to produce a significant amount of eggs are similar to the conventional In Vitro Fertilization (IVF). After the eggs are efficiently collected and identified by the embryologist, the Cumulus Oophorus, which is the outer layer of cells around each egg, is removed to view the egg without any obstructions. The eggs should be at a mature stage (M2) during the ICSI procedure. The procedure is thus accomplished with the use of a microscope using micromanipulation devices: micromanipulators, microinjectors, micropipettes. The mature oocyte is stabilized by a holding pipette on the left. Then, from the opposite side, a thin, hollow needle is pierced through the oolema and into the inner part of the oocyte. The needle is loaded with a single active sperm which will be released into the oocyte. On following day after the procedure, the oocyte is then checked for signs of fertilization.

ICSI Main Objective

The main objective of IVF specialist in performing ICSI is to minimize the rise of fertilization failure. At our centre, IVF specialized doctor uses ICSI back up to ensure fertilization in cases of unexplained infertility also. When the number of sperm is minute or has abnormal morphology or motility, it will result in the impairment in the function of the sperm. With ICSI, a single sperm is carefully and precisely placed within the egg.

ICSI Facts

Fertilization occurs in 50% to 80% of injected eggs. A total of 30% of all ICSI cycles performed results in live birth. According to an IVF doctor, younger patients are more probable to achieve favorable results from ICSI. Because ICSI enables infertile couples to achieve pregnancy and become parents of their own genetical child, it is a preferred method that is more prominent than Donor Insemination or Adoption.